Speakers: Stefania Cinquino, Florian Timo Ludwig, and Lisa Berman-Booty

The American College of Toxicology hosted a webinar focused on intrathecal and intracerebroventricular (ICV) dosing in non-clinical study design. The session featured presentations by Stefania Cecchino, Florian Ludwig, and Lisa Berman Booty, who shared insights on technical considerations, practical procedures, and microscopic findings associated with these delivery routes. Stefania discussed the benefits and challenges of IT and ICV dosing, emphasizing species-specific considerations and safety endpoints. Florian provided a detailed walkthrough of the lumbar puncture procedure in non-human primates, highlighting the importance of proper post-dosing observation and clinical neurological signs. Lisa focused on microscopic findings related to intrathecal administration, such as nerve fiber degeneration and inflammatory cell infiltrates, and offered strategies to differentiate procedure-related from test article-related effects. The speakers addressed questions on factors like flush volumes, dosing schedules, and imaging techniques to confirm correct administration sites. The webinar concluded with a reminder about upcoming ACT educational courses and the annual meeting.